In its simplest form, PCR based cloning is about making a copy of a piece of DNA and at the same time adding restriction sites to the ends of that piece of DNA so that it can be easily cloned into a plasmid …

Improve accuracy, increase efficiency and reduce errors with this handy guide to the plasmid cloning cycle.

Digest plasmid with the appropriate restriction enzymes to produce a DNA fragment that can be cloned directly into a vector. Unidirectional cloning is achieved with restriction enzymes that produce non …

Often, the first step in a molecular biology experiment is to clone (i.e. copy) a gene into a plasmid, then transform this recombinant plasmid back into bacteria so that essentially unlimited copies of the gene …

In a typical cloning experiment, a target gene is inserted into a circular piece of DNA called a plasmid. The plasmid is introduced into bacteria via a process called transformation, and bacteria carrying the …

Plasmids are a type of cloning vector. A vector is used to introduce DNA into cells. There are many types of vector, which is a more general term, and plasmids (the more specific term) are one type.

Feb 5, 2003 · During DNA cloning, a new gene is inserted into a loop of bacterial DNA called a plasmid. As shown in the animation, the plasmid is first cut with a restriction enzyme so that the gene of …