Protein yield and ease of purification are two important criteria in choosing a system for the expression/ purification of recombinant proteins. The IMPACT (Intein Mediated Purification with an ...

Immobilized metal affinity chromatography (IMAC) is a reliable method for isolation of recombinant protein following over-expression in Escherichia coli. The polyhistidine tag (6–10 consecutive amino ...

The host cell system is a critical determinant of recombinant protein quality and yield, with direct influence on protein folding and post-translational modification factors that shape the ...

The purification of proteins and antibodies is a critical process in biotechnology and pharmaceutical industries. Traditional methods often involve single-use materials, leading to high costs and ...

The baculovirus expression vector system (BEVS) provides a versatile platform for the expression of individual recombinant proteins as well as multimeric protein complexes, virus-like particles, ...